///2010 Abstract Details
2010 Abstract Details2018-05-01T17:52:49+00:00

Cardiac function by transthoracic echocardiography in healthy non pregnant and pregnant baboons (Papio hamadryas)

Abstract Number: 10
Abstract Type: Original Research

Alicia T Dennis Bachelor of Medicine and Bachelor of Surgery (MBBS), Postgraduat1 ; Annemarie Hennessy MBBS, PhD (University of Sydney), MBA exec, USMLE,2; Scott Heffernan Senior Animal Technician3

Introduction: Animal preparations of preeclampsia have been developed in the baboon however quantification of systolic and diastolic function by transthoracic echocardiography (TTE) has not previously been reported1. Understanding of these parameters prior to pregnancy and serially during induced preeclampsia is important in order to examine the effect of the disease on cardiac function and to examine changes that occur due to interventions. Baboons have similar anatomy, and are of comparable size to children. TTE devices used on the paediatric population can be applied to baboons. The use of a portable, non-invasive device has advantages in this research setting.

Methods: After institutional ethics approval, a standardised ketamine anaesthetic was administered to eight healthy non pregnant female baboons and six pregnant baboons. Manual blood pressure was recorded and using a paediatric cardiac probe TTE in the left lateral position parasternal long axis (PLAX), parasternal short axis (PSAX), apical 4- and 5- chamber (A4C, A5C) TTE including two-dimensional imaging and continuous, pulse wave and tissue Doppler was performed according to guidelines of the American Society of Echocardiography. Statistical analysis used the General Linear Model and unpaired t-test comparisons.

Conclusions: The non-invasive technique of TTE is applicable for the assessment of systolic and diastolic function in baboons. The administration of sedative anaesthesia and minimal disruption to the animals daily routine can enable serial cardiac function measurements under stable haemodynamic conditions. As invasive research and monitoring techniques are becoming less common in examining women with preeclampsia, the ability to use the same measuring device in humans and non-human primates enables better translation of animal results to human studies. This increases the relevance and applicability of animal studies to the human disease state.

References

1. Makris A et al Kidney Int 2007;71:977-84.



SOAP 2010